We aimed to explore the diversity and structural characteristics of rhizosphere bacteria associated with pecan plantations grown in three soil types (Luvisols, Cambisols, Solonchaks) in Eastern China and analyze their potential functions through high-throughput sequencing. However, whether soil type affects pecan (Carya illinoinensis) rhizosphere microbiomes is unclear. The rhizosphere microbiome is closely related to forest health and productivity. As a result, the community abundance of cbhI and GH48 genes were significant increased under combined application of crop residue and organic manure with chemical fertilizer condition.
There had significant positive correlation between soil humus C components (FA and HA) with abundance of cbhI and GH48 genes, and the o-alkyl C and AL% of FA were positively correlated with abundance of cbhI and GH48 genes. The results indicated that abundance of cbhI and GH48 genes in different particle-size fractions with RF and OM treatments were significant increased, compared with CK treatment. There had higher AL% and lower ARO% of FA and HA in different particle-size fractions with MF, RF, OM and CK treatments. Meanwhile, the alkyl C and Oalkyl C groups of FA and HA in > 2000 μm fraction with MF, RF, OM and CK treatments were higher than that of the other fractions. And the FA, HA and HM C contents in > 2000 μm and 2000–50 μm fractions with MF, RF and OM treatments were significant higher than that of CK treatment. The results showed that distribution of soil humus and cellulolytic microbial community abundance was significant increased under long-term application of crop residue and organic manure condition. The field experiment was included four different fertilizer treatments: chemical fertilizer alone (MF), rice straw and chemical fertilizer (RF), 30% organic manure and 70% chemical fertilizer (OM), and without fertilizer input as a control (CK). Therefore, the objective of this study were investigated the effects of 34-years long-term fertilizer regime on community abundance of cbhI and GH48 genes in five soil particle-size fractions (> 2000 μm, 2000–200 μm, 200–50 μm, 50–2 μm and 2–0.1 μm) by using polarization magic angle spinning 13C nuclear magnetic resonance spectroscopy. However, there is still limited information about how functional cellulose degradation response to long-term fertilizer management and their relative importance for C sequestration under the double-cropping rice paddy field in southern of China. There had close relationship between functional cellulose genes (cbhI and GH48) with characterize of soil organic matter chemical components (fulvic acid and humic acid) and soil physical fractions.
This suggests a link between actinobacterial GH48 community and soil organic carbon dynamics and highlights the potential importance of actinobacteria in the terrestrial carbon cycle.Ĭellulose plays an important role in maintaining or improving soil carbon (C) cycling and soil fertility of paddy field. Furthermore, mid infrared (MIR) spectrometry-predicted humic organic carbon was distinctly more important to GH48 diversity than to total bacterial and fungal diversity.
#GENEIOUS PRIME TUTORIAL DRIVER#
Soil C:N ratio was the primary environmental driver of GH48 communityĬomposition across sites and land uses, demonstrating the importance of substrate quality in their ecology. Phylogenetic analysis of the nucleotide sequences of 80 clones revealed significant new diversity of actinobacterial GH48 genes, and analysis of translated protein sequences showed that these are likely to represent functional cellobiohydrolases. For comparison, the diversity and abundance of general bacteria and fungi were also assessed. Here, the diversity and abundance of the actinobacterial glycoside hydrolase family 48 (cellobiohydrolase) gene wasĭetermined in soils from three paired pasture-woodland sites using T-RFLP and clone libraries with gene-specific primers. The role of actinobacteria in cellulose degradation has not been extensively investigated, despite their abundance in soil and known cellulose degradation capability. Cellulose accounts for approximately half of photosynthesis fixed carbon, however the ecology of its degradation in soil is still relatively poorly understood.
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